Total Phenolic Content of Organic and Conventionally Grown Gourd Vegetables
DOI:
https://doi.org/10.46243/jst.2020.v5.i4.pp166-172Keywords:
Total phenolics, Folin-Ciocalteu method, gourd vegetables, organic farming, ethanol,, methanol, water solventsAbstract
The increasing consumers’ demands to acquire healthier fruits and vegetables as well as the urgency in looking to natural compounds with antioxidant activity (AOA) has encouraged a quick expansion of research studies about phenolic in vegetables. Gourd vegetables refer to the fruits of plants in the two Cucurbitaceae genera Lagenaria and Curcubita. There is a diverse source of polyphenols in plant materials. Method: Seven gourd vegetables grown conventionally (CV) were collected from the local market and a set of the same vegetables from certified organic farms (OG). They were analysed for the total phenolic (TP) content by the Folin-Ciocalteu method using Gallic acid as standard. Earlier each vegetable was extracted with ethanol, methanol and water separately for the estimation of TP. The TP content was expressed as µg of GAE/ g of FW. Results: Bitter gourd had the highest amounts of TP in both the conventional (1766.52 µg of GAE/ g of FW)) and organic (1962.0 µg of GAE/ g of FW)) samples. Conventional gourd vegetables had more TP when compared to organic varieties. Among the solvents the extraction of phenolics was the highest in water followed by methanol and ethanol. Conclusion: Gourd vegetables are widely available and can contribute significant amounts of phenolics to the diet.
References
Crozier, A.; Jaganath, I.B.; Clifford, M.N., (2006), Phenols, polyphenols and tannins: An overview. In Plant Secondary Metabolites: Occurrence, Structure and Role in the Human Diet; Crozier, A., Clifford, M., Ashihara, H., Eds.; Blackwell: Oxford, UK, 2006; pp. 1-24
D”Archivio M, Filesi C, Vari R, Scazzocchio B, Masella R. (2010), Bioavailability of the polyphenols: status and controversies. Int J Mol Sci. 2010, 11:1321–42
Tsao R., (2010), Chemistry and biochemistry of dietary polyphenols. Nutrients. 2010, 2:1231–46. doi:10.3390/nu2121231
Jallali I, Megdiche W, M’Hamdi B, Oueslati S, Smaoui A, Abdelly C, Ksouri R., (2012), Changes in phenolic composition and antioxidant activities of the edible halophyte Crithmum maritimum L. with physiological stage and extraction method. Acta Physiologiae Plantarum. 2012, 34:1451–9. doi:10.1007/s11738-012-0943-9
Zhao F, Watanabe Y, Nozawa H, Daikonnya A, Kondo K, Kitanaka S: Prenylflavonoids and phloroglucinol derivatives from hops (Humulus lupulus). Journal of Natural Products. 2005, 68:43–9. doi:10.1021/np0499113
Sun B, Leandro MC, de Freitas V, Spranger MI., (2006), Fractionation of red wine polyphenols by solid-phase extraction and liquid chromatography. Journal of Chromatography A. 2006, 1128:27–38. doi:10.1016/j.chroma.2006.06.026
Greenfield, H. & Southgate, D.A.T., (2003), Food composition data: production, management, and use. London. Elsevier Applied Science
Garcia-Salas P, Morales-Soto A, Segura-Carretero A, Fernández-Gutiérrez A, (2010), Phenoliccompound-extraction systems for fruit and vegetable samples. Molecules. 2010, 15:8813. doi:10.3390/molecules15128813
Wong, P.Y.Y.; Kitts, D.D., (2006), Studies on the dual antioxidant and antibacterial properties of parsley (petroselinum crispum) and cilantro (coriandrum sativum) extracts. Food Chem. 2006, 97, 505–515. [CrossRef]
Rasheed AA, EI Cobham, M Zeighami, SP Ong, (2012), Extraction Of Phenolic Compounds From Pineapple Fruit, The 2nd International Symposium on Processing & Drying of Foods, Vegetables and Fruits (ISPDFVF 2012) University of Nottingham, Malaysia Campus, 18th–19th June 2012
Ainsworth EA, Gillespie KM., (2007), Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin-Ciocalteu reagent. Nat Protoc. 2007; 2(4):875–877
Armstrong, W.P., (1996), The Wild and Wonderful World of Gourds. Zoonooz 69 (10): 24-27
Koffi, E.; Sea, T.; Dodehe, Y.; Soro, S., (2010), Effect of solvent type on extraction of polyphenols from twenty three ivorian plants. J. Anim. Plant Sci. 2010, 5, 550–558
Budrat, P. and Shotipruk, A., (20080, Extraction of phenolic compounds from fruits of bitter melon (Momordica charantia) with subcritical water extraction and antioxidant activities of these extracts, Chiang Mai. Journal of Science, 35 (1), 123-130
Myojin, C., Enami, N., Nagata, A., Yamaguchi, T., Takamura, H., and Matoba, T., (2008), Changes in the radical scavenging activity of bitter gourd (Momordica charantia L.) during freezing and frozen storage with or without blancing. Journal of Food Science, 73 (7), 546-
Aminah, A. and Anna, P.K., ( 2011), Influence of ripening stages on physicochemical characteristics and antioxidant properties of bitter gourd (Momordica charantia), International Food Research Journal, 18 (3), 895-900
Kubola J. and Siriamornpun S., (2008), Phenolic contents and antioxidant activities of bitter gourd (Momordica charantia L.) leaf, stem and fruit fraction extracts in vitro. Food Chem., 2008 Oct 15;110(4):881-90. doi: 10.1016/j.foodchem.2008.02.076
Wu, S.J. and Ng, L.T., (2008), Antioxidant and free radical scavenging activities of wild bitter melon (Momordica charantia Linn. Var. abbreviates Ser.) in Taiwan. LWT-Food Science and Technology, 41: 323-330
Horax, R., Hettiarachy, N. and Islam, S., (2005), Total phenolic contents and phenolic acid constituents in four varieties of bitter melons (Momordica charantia) and antioxidant activities of their extracts, Journal of Food Science, 70 (4), c 275-c 280
Rezaeizadeh, A., Zuki, A.B.Z., Abdollahim N., Goh, Y.M., Noordin, M.M., Hamid, M. and Azmi, T.I.,(2011), Determination of antioxidant activity on methanolic and chloroformic extracts of Momordica charantia, African Journal of Biotechnology, 10 (24), 4932-4940
Isabella, M., Lee, B.L., Lim, M.T., Koh, W.P., Huang, D. and Ong, C.N., (2010), Antioxidant activity and profiles of common vegetables in Singapore, Food Chemistry, 120 (4), 993-1003
Sirohi, P.S., Choudary, B. and Kalda, T.S., (1991), Pumpkin “Pusavishwas” for tropical and subtropical region, Indian Horticulture, 36 (1), 24-26




