A PRELIMINARY STUDY ON DISINFECTION AND IN VITRO PROPAGATION OF CELOSIA TRIGYNA LINN.
DOI:
https://doi.org/10.46243/jst.2024.v9.i4.pp84-95Keywords:
Celosia, disinfection, explant, in vitro, MS, trigyna.Abstract
Celosia trigyna is an underutilized indigenous vegetable in Nigeria with numerous medicinal uses. Information is sparse on any attempt to conserve it. In other to conserve it through in vitro propagation, a disinfection protocol was developed using Clorox (NaClO) and Mancozeb (C8H12MnN4S8Zn) (Treatment T1 to T9) and a preliminary investigation testing its responsiveness in vitro. Nodal explants of Celosia trigyna were collected from the mother plants at Obafemi Awolowo University Estate, Ile – Ife (Latitude 7⁰32’N and Longitude 4⁰31’E). The nodal explants of C. trigyna were cultured on Murashige and Skoog (MS) medium supplemented with various levels of plant growth regulators (PGRs). The clean cultures obtained were monitored for degree of callus formation, morphology of callus, shoot and root regeneration. Treatment T6 (5% v/v Clorox for 9 minutes and 1% w/v mancozeb for 5 minutes) was best for disinfecting nodal explants of C. trigyna resulting into 81.47 ± 2.14a % aseptic cultures. Nodal explants cultured on MS with 0.5mg/L 2, 4 –D produced callus. This study established that using 5% v/v Clorox for 9 minutes followed by 1% w/v mancozeb for 5 minutes is effective for producing clean cultures of nodal explants and that C. trigyna can be propagated in vitro.
References
Andaryani S, Samanhudi S, and Ahmad Y. (2019). Effect of BAP and 2, 4-D on callus induction of Jatropha curcas. In vitro Cell Biology and Development, 3 (2): 56-65
Aregheore E. (2012). Nutritive Value and Inherent Anti-Nutritive Factors in Four. Indigenous Edible Leafy Vegetables in Human Nutrition in Nigeria: Journal of Food Resource Science 1: 1-14
Bakar A, Dalilah A, Bakrudeen A, Taha M, Rosna MT. (2014). In vitro Callus Induction and Plant Regeneration of Celosia argentea–an Important Medicinal Plant. Brazilian Archives of Biology and Technology 57(6): 860-866
Denton OA (2004). Celosia trigyna L. [Internet] Record from PROTA4U. Grubben, G.J.H. & Denton, O.A. (Editors). PROTA (Plant Resources of Tropical Africa / Ressources végétales de l’Afrique tropicale), Wageningen, Netherlands. < http://www.prota4u.org/search.asp>
El-Shafey N, Sayed M, Ahmed E, Hammouda O, Khodary SE. (2019). Effect of growth regulators on micropropagation, callus induction and callus flavonoid content of Rumex pictus Forssk. Egyptian Journal of Botany 59(2): 293-302
George EF, Hall MA, De Klerk, GJ. (2008) Plant Propagation by Tissue Culture. 3rd Edn., Springer, Dordrecht, Netherlands, Pp 501
Gomez KA, Gomez AA (1984). Statistical Procedures for Agricultural Research. 2nd Edn., Wiley, Newyork, Pp 643
Hemmati N, Cheniany M, Ganjeali A. (2020). Effect of plant growth regulators and explants on callus induction and study of antioxidant potentials and phenolic metabolites in Salvia tebesana Bunge. Botanica Serbica 44(2): 163-
Mabona U, Van, Vuuren S, Vikjoen A, Shikanga E. (2013). Antimicrobial Activity of Southern African Medicinal Plants with Dermatological Relevance: From an Ethnopharmacological Screening Approach to Combination Studies and the Isolation of a Bioactive Compound. Journal of Ethnopharmacology 148(1): 45-55
Murashige T, Skoog F. (1962). A Revised Medium for Rapid Growth and Bioassays with Tobacco Tissue Cultures. Plant Physiology 15: 473-497
Ofusori A, Moodley R, Jonnalagadda S (2018). Elemental Distribution in the Edible Leaves of Celosia trigyna from the Western and Northern Regions of Nigeria. Journal of Environmental Science and Health, Part B 54 (1): 61-69
Ogbimi E, Sakpere A, Ayisire B. (2016). Effect of Naphtalene Acetic Acid and Benzylaminopurine Ejeoghene R. OGBIMI, Ayobola M. SAKPERE, Michael S. AKINROPO, Seyi E. ADEBOYE3: A PRELIMINARY STUDY ON DISINFECTION AND IN VITRO PROPAGATION OF CELOSIA TRIGYNA LINN. on In vitro Propagation of Solanecio biafrae–A Threatened Indigenous Leafy Vegetable. FUTA Journal of Research in Sciences 12(2): 211-218
Orwa C, Mutua A, Kindt R, Jamnadass R, Anthony S. (2009). Tropical Plants Database, Ken Fern. tropical.theferns.info.Assessed on 2020-06 21. Agroforestry Database: a tree reference and selection guide version 4.0. World Agroforestry Centre, Kenya
Saurabh B, Kiran S, Randhir D, Tanmoy B. (2015). Modern Application of Plant Biotechnology in Pharmaceutical Science. ISBN 978-0-12-8022214
Sowemimo A, Van de Venter M, Baatjies L, Koekemoer T. (2009). African Journal of Traditional and Complement Alternative Medicine 6: 526
Yan Y et al. (2014). Effect of Naphtalene Acetic Acid on Adventitious Root Development and Associated Physiological Changes in Stem Cutting of Hermathria compressa. PLoS ONE 9(3): e90700. https://doi.org/10.1371/journal. pone.0090700
